【摘 要】
：
稻瘟病菌(Magnaporthe oryzae)是研究植物宿主与病原菌之间相互作用关系的一种模式真菌,其主要侵染水稻,造成大量减产.Chm1是一类丝氨酸/苏氨酸蛋白激酶PAK家族的一员,PAKs通常作为Rho GTP酶的效应蛋白起作用.研究证明,酵母中Chm1同源基因Ste20与Cdc42互作,共同参与调控菌丝的生长和信息素反应等.稻瘟病菌中MoChm1通过一个高度保守的P21结合区域(PBD d
【机 构】
：
福建农林大学生命科学学院,福州 350002 福建农林大学植物保护学院,福州 350002
【出 处】
：
中国植物病理学会2014年学术年会
论文部分内容阅读
稻瘟病菌(Magnaporthe oryzae)是研究植物宿主与病原菌之间相互作用关系的一种模式真菌,其主要侵染水稻,造成大量减产.Chm1是一类丝氨酸/苏氨酸蛋白激酶PAK家族的一员,PAKs通常作为Rho GTP酶的效应蛋白起作用.研究证明,酵母中Chm1同源基因Ste20与Cdc42互作,共同参与调控菌丝的生长和信息素反应等.稻瘟病菌中MoChm1通过一个高度保守的P21结合区域(PBD domain)与小G蛋白Rac1结合,对于调节稻瘟病菌的产孢途径有着重要的作用.因此为了进一步说明产孢途径中Rac1-Chm1这条信号途径,我们利用RNA-sequence和iTRAQ技术对MoChm1缺失突变体进行了研究.结果表明,相对野生型Guy11,有部分基因在MoChm1缺失突变体产孢过程中RNA与蛋白水平产生不同程度的上调与下调,从中筛选出3个上调或下调较明显的基因,分别为MoUbr1(上调8倍)、MoFre(下调16倍)和MoCdc3(上调2倍),并对其逐一进行敲除.表型分析结果显示,相较于Guy11,MoUbr1和MoFre敲除突变体在生长、产孢和致病性等表型上均没有明显的差异,但MoCdc3敲除突变体生长速度下降,产孢量极低,出现单隔膜和无隔膜的异常分生孢子,而以上这些表型都与MoChm1缺失突变体类似.通过Pull Down验证,MoCdc3与MoChm1存在互作关系,因此MoCdc3极有可能是MoChm1假定的下游靶标.
其他文献
Fusarium verticillioides is one of the most important fungal pathogens to cause destructive diseases of maize worldwide.Fumonisins produced by the fungus are harmful to human and animal health.In Fusa
Puccinia triticinia (Pt) is an obligate parasite pathogen which caused wheat leaf rust all over the world, and causing significant yield losses in wheat production when the diesease developed seriousl
Fusarium head blight (FHB) mainly caused by Fusarium graminearum is a devastating disease of cereal crops worldwide.The conidia produced by the fungal pathogen F.graminearum are the main source of dis
Wheat stem rust was once the most destructive though it has been successfully under control mainly through genetic methods guided by the information of close surveillance of race and virulence of the
Expert system has eveloped to crop breeding, fertilization, irrigation, diseases diagnosis, prevention and other fields.The central issues of the expert system is to solve the objectives optimization
Konjac (Amorphaphallus B1.ex Decne) is an important economic crop in Asia.In 2012, konjac phytophthora blight (KPB) were observed in the field, fuyuan country, yunnan province, China.The pathogen caus
In this study, 36 soil samples were collected from tobacco filed in Songxian, Luoning and Ruyang counties of Luoyang.Through isolation and purification, 27 isolates of Trichoderma were obtained.7 isol
Subl/PC4, the well-known general transcription cofactor, has multiple functions in transcription and DNA repair.Here we present the crystal structure at 2.7(A) resolution of MoSub1, the Sub1/PC4 ortho
Blueberry (Vaccinium corymbosum) was becoming widely cultivated in recent years in China.On september 2013, stem blight of blueberry was observed on blueberry in plantation in Changting, Fujian provin
Magnaporthe oryzae, the causal agent of rice blast, has been used as a model for investigating the interaction between phytopathogenic fungi and plants.Identification genes involved in mycelial growth