Evidence have shown that estrogen is not only synthesized in gonadal glands but also in hippocampus,and its role in modulate synaptic plasticity which is the basic ability of learning and memory,has long been explored.The signal pathway whereby estrogen modulate synaptic plasticity consists of a genomic pathway mediated by ERαand ERβand a non-genomic pathway mediated by GPR30,and its not clear which pathway play a dominant role in nervous system.In our previous study,EtOAc extract of Cynomorium Songaricum(ECS)can improve both E2 level and the ability of learning and memory in ovariectomized rat.Therefore we suppose it has an estrogen-like effect.To discuss the possible pathway that ECS improve ability of learning and memory,we block genomic signal pathway on Neuro2a cells with Tamoxifen,agonist for GPR30 while antagonist for ERαand ERβ,using western blots to detect the expression of which is closely related to synaptic plasticity,and immunofluorescence to show the location.The results of westernblots show that ECS upgrades the expression of synaptophysin compared with the normal(p<0.05).In addition,the expression of snaphytophysin in ECS+tamoxifen group >tamoxifen group(p<0.05).The immunofluorescence image show that synaptophysin located in the cytoplasm.These findings suggest that ECS participates in the modulation of synaptic plasticity potentially via a GPR30 signal pathway.In further study,we need to detect the downstream of non-genomic estrogen pathway to facilitate our understanding of ECS,which will be very important to develop a new strategies of estrogen replacement therapy against neurodegenerative diseases.