Purpose: To evaluate the effect of osteogenic and angiogenic bi-lineage differentiated adipose tissuederived stem cells(ADSCs)sheet on implant osseointegration in the irradiated bone.Methods: The lower hindlimbs of rats had external irradiation of a single dose of 20Gy.Eight weeks later,osteogenic and angiogenic bi-lineage differentiated ADSCs sheet-implant complex(Group A)was implanted in the tibia of the irradiated rat.Osteogenic differentiated ADSCs sheet-implant complex(Group B)and traditional pure Ti implant(Group C)were served as control groups.After eight weeks of implantation,Micro CT assay were conducted in vivo.Then the pull-out tests were proceeded and the rest of specimens were utilized to make hard tissue slices to analyze the implant osseointegration.