【摘 要】
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Objective Downregulating the expression of VEGF expression with low-frequency ultrasound and microbubbles, explore the synergy effect of low-frequency ultrasound and immune cells phenotype in anti-tum
【机 构】
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Department of Ultrasound in Medicine,Shanghai Jiaotong University Affiliated 6th People's Hospital,
【出 处】
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第三届全国暨国际超声分子影像及生物效应和治疗学术会议
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Objective Downregulating the expression of VEGF expression with low-frequency ultrasound and microbubbles, explore the synergy effect of low-frequency ultrasound and immune cells phenotype in anti-tumor function of PCa microenvironment, thus providing a potential new method for PCa therapy.Methods To study the influence of ultrasound and microbubbles on VEGF expression in murine PCa cells RM-1, the impact of ultrasonic frequency, ultrasonic power, irradiation time and micro-bubble/cell suspension volume ratio were investigated according to orthogonal design.The injured RM-1 cells were observed by Typan blue staining immediately, and western blot was adopted to detect the expression of VEGF after another 24 hours cultivation.
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