Purpose: To study the effects of different radiation quality on Warburg Effect.Materials and methods: HeLa cells were irradiated by 12C6+ ions and X-rays with a dose of 2 Gy.1 hour after treated, concentrations of lactate and ATP were measured by using lactate and ATP determination kits.Results: The level of ATP was all increased after 12C6+ and X-ray irradiation.But lactate concentrations were not altered after 12C6+ irradiation, while ascended after exposed to X-rays.Conclusions: The data indicate that there were different energy metabolism patterns in responding to 12C6+ ion beam and X-rays.To 12C6+ ions, the increased ATP level maybe came from the rising of aerobic respiration, but to X-rays, the rising concentrations of ATP and lactate was mainly from glucolysis.