Both brain ageing and Amyloid beta protein (Aß) is known to be associated with the decrease of membrane fluidity and the overload of intracellular calcium ([Ca2+]i).Aniracetam is a known nootrop
Objective Using laser-Doppler flowmetry (LDF),the influence of tetramethylpyrazine (TMP) on regional cerebral blood flow (rCBF) of temporal cortex in rat after focal cerebral ischemia/reperfusion inju
目的 为了探讨人谷氨酸脱羧酶(hGAD65)在PD治疗中的作用,拟克隆hGAD65基因,将其重组在腺相关病毒载体(rAAV)上,并在体外检测其功能,为体内实验积累数据.方法结果 取流产胚胎的脑组织(海马、皮质)应用RT-PCR的方法获得GAD65的cDNA,连于T-easy载体上.测序得到结果与genebank中的GAD65基因的一致率达到98％,几个突变点并没有产生错意突变.应用双酶切将GAD6
目的 研究K141N HSPB8细胞内分布特点及41N HSPB8与HSPB1、NFL的共定位分析.方法 建立瞬时表达GFP-HSPB8和GFP-K141N HSPB8的SHSY-SY细胞、Hela细胞和HEK293T细胞模型，在激光共聚焦显微镜下观察GFP-K141N HSPB8聚集物形成情况，采用t检验和单因素方差分析的统计学方法分析聚集物形成的可能机理.运用间接免疫荧光染色进行GFP-K14
MPTP(1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine)可以使灵长类动物产生帕金森病样症状和病理改变,且这些症状可以被左旋多巴缓解,因此被广泛用作帕金森病动物模型造模工具药。目前常用的MPTP模型为小鼠的急性和亚急性模型。本实验旨在研究MPTP小鼠慢性模型以更好模拟帕金森病的慢性进行性发展的病程特点。实验结果表明,MPTP低剂量腹腔注射,每日一次,连续给
Early-life exposure to SSRI antidepressants increases the chances of developing mood disorders later in life.However,the biological basis in term of neuronal morphology for these behavioral changes is
Objective To investigate the neuroprotective actions of pioliotazone (Piog) and the effects of Piog-mediated PPART activation on NF-B signaling pathway in rat model of permanent focal cerebral ischemi
Cysteinyl leukotrienes (CysLTs) are 5-1ipoxygenase (5-LOX) metabolites of arachidonic acid and exert potent pro-inflammatary effects via their receptors including cysteinyl leukotriene receptors 1 and
Objective Pelizaeus-Merzbacher disease (PMD; OMIM 312080) is a rare X-linked recessive disorder presenting with nystagmus,impaired motor development,ataxia,and progressive spasticity.Traditionally,PMD
Congenital motor nystagmus (CMN) is a relatively common oculomotor disorder characterized by bilateral uncontrollable ocular oscillations.Recently,the FRMD7 gene mutation has been identified as the ge