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Penthorum chinense Pursh.is a traditional Chinese herbal medicine and used for the treatment of various ailments specially related to liver.Gansu Granule,the medicine made from the extract of P.chinense,has been widely used in the clinical settings.But the information about its active ingredients is lacking.In this paper,the extract of P.chinense was analyzed by HPLC-ESI-FT-ICR-MS/MS.