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植物在受植食性昆虫为害时能产生防御反应,并且植物的茉莉酸信号转导途径在这一过程中发挥着重要作用。然而,迄今为止对于油菜Brassica campestris的诱导防御反应很少有研究报道。为此,本实验通过测定油菜内茉莉酸和胰蛋白酶抑制剂含量研究了油菜在斜纹夜蛾Spodoptera litura幼虫为害后的抗虫性和胰蛋白酶抑制剂含量的变化,并分析这些变化在油菜诱导抗虫性与茉莉酸信号转导途径中的关系。结果表明:斜纹夜蛾幼虫取食能导致油菜体内茉莉酸和胰蛋白酶抑制剂含量系统性上升,外用茉莉酸甲酯处理也能系统性增加油菜的胰蛋白酶抑制剂含量,并且取食茉莉酸甲酯处理或斜纹夜蛾幼虫取食过的叶片能显著降低斜纹夜蛾幼虫的体重,两者的体重分别为对照植株上的67.5%和60.2%。机械损伤加斜纹夜蛾幼虫口腔分泌物处理能引起处理叶中茉莉酸和胰蛋白酶抑制剂含量的增加,但其诱导效果与机械损伤加水没有显著差异,并且两者明显低于虫害的诱导效果,两种处理的茉莉酸和胰蛋白酶抑制剂含量分别为虫害诱导的68.4%和24.4%及62.9%和36.9%;多次连续机械损伤的诱导效果与一次损伤的没有明显差异。结果说明斜纹夜蛾幼虫诱导的油菜抗虫性与茉莉酸信号转导途径有关,而其激活油菜抗虫反应的机理则可能与其特定的取食行为相关。
Plants produce defensive responses to phytopathogenic insects, and the plant’s jasmonic acid signal transduction pathway plays an important role in this process. However, little has been reported so far on the induced defense response to Brassica campestris. Therefore, in this experiment, we studied the changes of pest resistance and trypsin inhibitor content in rapeseed (Spodoptera litura) larvae by measuring the content of jasmonic acid and trypsin inhibitor in rapeseed. We also analyzed these changes in rapeseed induced resistance Relationship between insect and jasmonic acid signal transduction pathways. The results showed that the feeding of E. litura larvae resulted in a systematic increase of jasmonic acid and trypsin inhibitor content in rapeseed. Topical application of methyl jasmonate could also increase the content of trypsin inhibitor in rapeseed, Ester-treated or S. litura larvae fed on leaves significantly reduced the body weight of S. litura larvae, both at 67.5% and 60.2% of control plants, respectively. Mechanical damage plus oral cavity worms caused by Spodoptera litura could increase the content of jasmonic acid and trypsin inhibitor in leaves, but its inducing effect was not significantly different from that of mechanical injury plus water, and both were significantly lower than those induced by pests. The contents of jasmonic acid and trypsin inhibitor in the two treatments were 68.4% and 24.4% and 62.9% and 36.9% of those induced by pest insects respectively. There was no significant difference in induction effect between multiple continuous mechanical injuries and one injury. The results showed that the resistance of B. litura larvae induced by rape was related to the jasmonic acid signal transduction pathway, and its mechanism of activating pest resistance may be related to its specific feeding behavior.