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采用毛细管凝胶电泳技术对燕窝及银耳中的水溶性蛋白质进行分离测定。通过优化条件,建立燕窝、银耳的蛋白提取方法以及毛细管凝胶电泳方法,检测燕窝和银耳水溶性蛋白标志峰。同时采用3种燕窝样品对所建立的方法进行验证,证实了方法的可靠性。毛细管电泳技术对燕窝和银耳水溶性蛋白定量准确,重复性、精密度、稳定性各项指标良好,适合于对掺假燕窝中银耳成分的检测。
Capillary gel electrophoresis was used to determine the water-soluble proteins in the nest and Tremella. By optimizing the conditions, the establishment of bird’s nest, white fungus protein extraction method and capillary gel electrophoresis method, detection of bird’s nest and white fungus water-soluble protein marker peak. At the same time using three kinds of bird’s nest samples of the established method to verify that the reliability of the method. Capillary electrophoresis technology for quantitative determination of bird’s nest and white fungus water-soluble protein, repeatability, precision and stability of the indicators are good, suitable for the adulterated bird’s nest Tremella composition detection.