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目的:观察汉滩病毒感染血管内皮细胞(EVC304)前后,HLA-I类分子中HLA-A、HLA-B、HLA-Cw3、HLA-Cw5表达上的差异。方法:以汉滩病毒76-118株感染EVC304细胞作为实验组,以无任何刺激的EVC304细胞作为阴性对照组。6h后用RT-PCR的方法对HLA-A、HLA-B、HLA-Cw3、HLA-Cw5的mRNA进行反转录和基因片段的扩增,在mRNA水平上检测其表达差异。结果:在HTNV感染以后,EVC304细胞中HLA-A、HLA-B、HLA-Cw3、HLA-Cw5mRNA表达均呈升高现象,其中HLA-A、HLA-B和HLA-Cw5的mRNA表达与未感染细胞相比明显升高。结论:HTNV感染血管内皮细胞后,可以诱导HLA-A、HLA-B、HLA-Cw3、HLA-Cw5的表达升高,从而诱发机体特异性免疫应答,导致免疫损伤的发生。这或许是肾综合症出血热的发病机制之一。
Objective: To observe the differences of HLA-A, HLA-B, HLA-Cw3 and HLA-Cw5 expression in Hantaan virus infected vascular endothelial cells (EVC304). Methods: EVC304 cells were infected with Hantaan virus strain 76-118 as experimental group and EVC304 cells without any stimulation as negative control group. The expression of HLA-A, HLA-B, HLA-Cw3 and HLA-Cw5 mRNA was reverse transcribed and the gene fragment was amplified by RT-PCR 6h later. Results: After HTNV infection, the expression of HLA-A, HLA-B, HLA-Cw3 and HLA-Cw5 mRNA increased in EVC304 cells, and the mRNA expression of HLA-A, HLA- Cells were significantly higher than that. CONCLUSION: HTNV can induce the expression of HLA-A, HLA-B, HLA-Cw3 and HLA-Cw5 after being infected by vascular endothelial cells, which can induce the body-specific immune response and lead to the occurrence of immune damage. This may be one of the pathogenesis of hemorrhagic fever with renal syndrome.