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作者首次应用4种咪唑类药物,观察对杀灭人巨噬细胞物内的热带利什曼原虫的作用,选择从伊朗分离的NIH株173的无鞭毛期热带利什曼原虫,按6:1的比例感染从正常人末梢血中取出的已在试管内培养6天的单核细胞,分成加抗利什曼药物的实验组及不加药物的对照组,两组均加RPMI-1640培养基,青霉素50单位/ml,链霉素50μg/ml,10%灭活的胎牛血清。3天换1次培养基,感染后5～6天,取出各组的培养物固定染色和镜检,分别计数100个巨噬细胞中的利什曼小体的平均数的百分率。结果克霉唑
The authors first applied four kinds of imidazole drugs to observe the role of killing Leishmania leishmanii in human macrophages in vivo, selecting NIH strain 173 isolated from Iran, Were infected with monocytes which had been cultured in vitro for 6 days and were divided into two groups: experimental group with anti-Leishman drug and control group without drug, and both groups were supplemented with RPMI-1640 medium 50 units / ml penicillin, 50 μg / ml streptomycin, 10% inactivated fetal bovine serum. 3 days for a medium, 5 to 6 days after infection, remove the culture fixed staining and microscopic examination of each group, were counted 100 macrophages Leishmania average percentage. Results Clotrimazole