论文部分内容阅读
应用改良的DEAE－Fractogel阴离子交换法生产高纯度FⅧ，并经过TNBP和Tween－80灭活病毒，使单位体积凝胶交换容量增加20％，并改进了冻干制品的溶解性和稳定性，FⅧ活性保持30IU／ml以上，比活性达到200IU／ml蛋白，安全性可靠，适合于大规模生产。
High purity FⅧ was produced by modified DEAE-Fractogel anion exchange method and inactivated by TNBP and Tween-80 to increase the gel exchange capacity per unit volume by 20% and to improve the solubility and stability of lyophilized products. FⅧ Activity to maintain 30IU / ml or more, the specific activity of 200IU / ml protein, safe and reliable, suitable for mass production.