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本文首次建立了广东王不留行活性成分水解后槲皮素的含量测定方法,为评价广东王不留行的质量和制定其质量标准提供依据。采用HPLC-UV法,以C18反相色谱柱,甲醇–0.4%(v/v)磷酸溶液(50:50,v/v)为流动相等度洗脱,检测波长为360nm。对11批广东王不留行样品进行测定,实验证明该含量测定方法分离效果好、灵敏度高、重复性好,平均回收率为99%,可作为广东王不留行的质量控制方法。
In this paper, the method for the determination of quercetin in the hydrolyzate of the active ingredients of Guangdong Wangbuyu was established for the first time to provide the basis for the evaluation of the quality of the Guangdong Wangbuyonghang and the formulation of its quality standards. The mobile phase consisted of C18 reversed-phase column and methanol-0.4% (v / v) phosphoric acid solution (50:50, v / v). The detection wavelength was 360 nm. The determination of 11 batches of samples from Guangdong Wangfuqing Bank was carried out. The experiments show that the method has good separation effect, high sensitivity and good repeatability, and the average recovery rate is 99%. It can be used as the quality control method of Guangdong Wangbuwuhang Bank.