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目的 探讨眼部鳞状细胞癌中细胞黏附分子变异体 6(clusterofdifferentiation 44variant 6,CD4 4V6 )表达及与增殖细胞核抗原 (proliferatingcellnuclearantigen ,PCNA)的关系。方法 应用链霉亲和素 生物素 过氧化物酶复合物免疫组化法检测 3 5例眼部鳞状细胞癌、2 0例乳头状瘤和 1 1例正常眼睑皮肤组织标本CD4 4V6 蛋白表达。结果 CD4 4V6 蛋白在眼部鳞状细胞癌 (squamouscellcarcinoma,SCC)的阳性表达率为 62 9% ( 2 2 / 3 5) ,而在乳头状瘤的阳性表达率为 1 5 0 % ( 3 / 2 0 ) ,在 1 1例正常眼睑皮肤组织均无CD4 4V6 蛋白的阳性表达 ;恶性组CD4 4V6 蛋白的阳性表达率明显高于良性组 ( χ2 =1 1 57,P <0 0 1 )和正常组 (P =0 0 0 1 ) ,8例淋巴转移的CD4 4V6 蛋白的阳性表达率明显高于无转移者 (P =0 0 49) ;CD4 4V6 蛋白阳性表达者其PCNA指数明显高于阴性表达者组 ,差异有统计学意义 (t=2 0 2 1 ,P <0 0 1 )。结论 CD4 4V6 分子可能与眼部鳞状细胞癌的恶性表型、浸润及转移有关 ,CD4 4V6 蛋白的表达与PCNA蛋白表达具有正相关性 ,二者的同时检测对评估患者的预后可能有临床参考价值。
Objective To investigate the relationship between the expression of cluster 4 differentiation-type 6 (CD4 4V6) and the proliferating cell nuclear antigen (PCNA) in ocular squamous cell carcinoma. Methods The expression of CD4 4V6 protein in 35 cases of ocular squamous cell carcinoma, 20 cases of papilloma and 11 cases of normal eyelid skin specimens was detected by streptavidin biotin peroxidase complex immunohistochemistry. Results The positive rate of CD4 4V6 protein in squamous cell carcinoma (SCC) was 62.9% (2 2/3 5), while that in papilloma was 150% (3/2) 0). The positive rate of CD4 4V6 protein expression in malignant group was significantly higher than that in benign group (χ2 = 1157, P <0.01) and normal group (P = 0.00001). The positive rate of CD4 (4) V6 protein in 8 cases with lymph node metastasis was significantly higher than that without metastasis (P = 0 0 49). The PCNA index of CD4 4V6 positive group was significantly higher than that of negative ones Group, the difference was statistically significant (t = 2 0 2 1, P <0 0 1). Conclusions CD4 4V6 may be involved in the malignant phenotype, invasion and metastasis of squamous cell carcinoma of the eye. The expression of CD4 4V6 protein is positively correlated with the expression of PCNA protein. The simultaneous detection of CD4 4V6 and MMP6 may have clinical significance in evaluating the prognosis of patients value.