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目的初步探讨中期阿尔茨海默病(AD)的presenilin-1/presenilin-2双基因条件性敲除小鼠(d KO mice)模型中海马组织Atp5a1基因的甲基化改变情况。方法运用简化的表观亚硫酸盐测序技术(RRBS)检测3只12月龄雌性d KO mice和3只同系同龄雌性野生型小鼠海马组织基因组DNA异常甲基化情况,利用Bismark(v O.7.4)软件进行对照分析获取异常甲基化基因。结果二代测序结果显示12月龄中度神经退行性病变AD d KO mice海马中Atp5a1基因呈低甲基化状态(P<0.05)。结论 Atp5a1基因在中期AD d KO mice的海马中呈低甲基化状态,提示低甲基化的Atp5a1基因可能作为参与中期AD病程的潜在候选基因。
Objective To investigate the methylation of the Atp5a1 gene in hippocampus of presenilin-1 / presenilin-2 double gene knockout mice (d KO mice) model in mid-term Alzheimer’s disease (AD). Methods The abnormal methylation of genomic DNA in hippocampus of three 12-month-old female d KO mice and 3 same-age female wild-type mice was detected by the simplified Apparent Sulfite Sequencing (RRBS) technique. Bismark (v. 7.4) Control software to obtain abnormal methylation genes. Results The results of second generation sequencing showed that the Atp5a1 gene in hippocampus was hypomethylated (P <0.05) in 12-month-old moderate neurodegenerative lesions. Conclusions Atp5a1 gene is hypomethylated in hippocampus of mid-term AD d KO mice, suggesting that hypomethylated Atp5a1 may serve as potential candidate genes involved in mid-AD disease.