利用马铃薯X病毒(Potato virus X,PVX)异源病毒载体在本氏烟中分别和共同表达了南方水稻黑条矮缩病毒可能编码的6种非结构蛋白(Pns52、Pns6、Pns71、Pns72、Pns91和Pns92),采用实时荧光定量PCR技术分析了其表达水平。结果表明,与PVX空载体侵染的本氏烟相比,只有Pns92蛋白的表达能够表现出典型的花叶症状,6种蛋白共同表达时的症状与Pns92单独表达呈现的症状相似。推测Pns92可能与病毒的致病性有关。 The six non-structural proteins (Pns52, Pns6, Pns71, Pns72, Pns91, Pns91, Pns91 and Pns91) that could be encoded by southern rice black-streaked dwarf virus were expressed separately and co-expressed in N. benthamiana using heterologous viral vectors of Potato virus X And Pns92), and their expression levels were analyzed by real-time fluorescence quantitative PCR. The results showed that compared with N. benthamiana infected with PVX empty vector, only the expression of Pns92 protein showed typical mosaic symptoms. The expression of six proteins were similar to that of Pns92 alone. Speculated Pns92 may be related to the virulence of the virus.