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以水稻“爱知旭”（Ａｉｃｈｉａｓｈａｈｉ）为寄主，将带选择标记的质粒ｐＣＳＮ４３和ｐＢＦ１０１为外源ＤＮＡ，利用限制酶诱导整合（ＲＥＭＩ）这种新方法转化稻瘟病菌原生质体，从筛选到的数百个转化体中分离出３个与致病能力密切相关的突变体Ｒ２Ｈ６５、Ｒ２Ｈ６９和Ｒ２Ｂ１５６５。其中，Ｒ２Ｈ６５和Ｒ２Ｈ６９只产生畸形分生孢子，分生孢子的发育和附着胞的形成以及黑色素的合成均受到极大影响，致病性测试证明完全丧失致病能力；Ｒ２Ｂ１５６５的许多表型与野生型的相似，但致病能力却大大降低。
Using the rice Aichi-Ashahi as host, the selected marker plasmids pCSN43 and pBF101 were used as exogenous DNA and transformed into Magnaporthe grisea protoplasts by restriction enzyme-induced integration (REMI) Among the hundreds of transformants, three mutants R2H65, R2H69 and R2B1565 which are closely related to pathogenicity were isolated. Among them, R2H65 and R2H69 produced only conidia of conformation, the development of conidia and the formation of adherent cells and the synthesis of melanin were greatly affected, and the pathogenicity test proved the complete loss of pathogenicity. Many phenotypes of R2B1565 and wild Similar, but greatly reduced pathogenic capacity.