Objective:To review the diagnosis of chronic wound biofilms and discuss current treatment approaches.Data sources:Articles included in this review were obtained from the following databases:Wanfang,China National Knowledge Infrastructure,PubMed,and the Web of Science.We focused on research published before August 2019 with keywords including chronic wound,biofilm,bacterial biofilms,and chronic wound infection.Study selection:Relevant articles were selected by carefully reading the titles and abstracts.Further,different diagnosis and clinical treatment methods for chronic wound biofilm were compared and summarized from the selected published articles.Results:Recent guidelines on medical biofilms stated that approaches such as the use of scanning electron microscopy and confocal laser scanning microscopy are the most reliable types of diagnostic techniques.Further,therapeutic strategies include debridement,negative pressure wound therapy,ultrasound,antibiotic,silver-containing dressing,hyperbaric oxygen therapy,and others.Contusion:This review provides the identification and management of biofilms,and it can be used as a tool by clinicians for a better understanding of biofilms and translating research to develop best clinical practices.