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为确定肺结核的诊断及考核疗效,痰结核菌的检查是非常重要的。为探讨镜检时间对检出率的影响,我们对1800份门诊肺结核患者的痰标本,分别统计1～10分钟的镜检痰菌检出情况,以便找出合适的镜检时间。一、标本来源:收集门诊初、复治肺结核患者清晨第一口痰标本共1800份。二、试验方法:采用厚涂片抗酸染色法。三、镜检:每分钟平均看20个视野。四、结果:以Gaffky氏法进行计数,分别将镜检1～10分钟阳性数进行登记,10分钟后未找到者,即报告未发现结核菌(结果见表一、二)。
In order to determine the diagnosis and assessment of tuberculosis, the examination of sputum TB is very important. In order to explore the effect of microscopic examination time on the detection rate, we detected the sputum samples of sputum from 1 to 10 minutes of sputum samples of 1800 outpatients with tuberculosis in order to find out the suitable microscopic examination time. First, the specimen source: early outpatient, retreatment tuberculosis patients first sputum specimens a total of 1800 copies. Second, the test method: acid-resistant thick stain smear method. Third, microscopic examination: an average of 20 views per minute. Fourth, the results: The Gaffky’s method of counting, respectively, the positive number of microscopy 1 to 10 minutes to register, not found after 10 minutes, that is, the report did not find TB (results in Table I, II).