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目的研究肺癌血液播散的实验诊断方法。方法监测肺癌患者外周血癌胚抗原mRNA(CEA mRNA),细胞角蛋白19 mRNA(CK19 mRNA),端粒酶活性(TA)和癌胚抗原(CEA)表达的变化,评估检测这些标志实验诊断肺癌血液播散的价值。结果CEA mRNA,CK19 mRNA,TA和CEA在肺癌患者外周血的阳性率显著高于良性肺病变患者和健康者(P<0.01),其中CEA mRNA和TA的阳性率显著高于CK19 mRNA和CEA(P<0.01),且在健康者外周血未见表达,在良性肺病变患者外周血阳性率低于CK19 mRNA和CEA,CEA mRNA和TA阳性者的生存期明显短于阴性者(P<0.01)。结论检测外周血中CEA mRNA,CK19 mRNA,TA和CEA可用于监测肺癌外周血播散,CEA mRNA和TA比CK19 mRNA和CEA的效果更好。
Objective To study the method of experimental diagnosis of disseminated lung cancer. Methods The changes of CEA mRNA, CK19 mRNA, telomerase activity (TA) and carcinoembryonic antigen (CEA) expression in peripheral blood of patients with lung cancer were measured. The levels of CEA mRNA, CEA, Spread the value. Results The positive rates of CEA mRNA, CK19 mRNA, TA and CEA in peripheral blood of patients with lung cancer were significantly higher than those of patients with benign lung disease and healthy subjects (P <0.01). The positive rates of CEA mRNA and TA were significantly higher than those of CK19 mRNA and CEA P <0.01). The positive rate of peripheral blood in patients with benign pulmonary disease was lower than that of CK19 mRNA and CEA, and the survival rate of CEA mRNA and TA positive patients was significantly shorter than that of negative patients (P <0.01) . Conclusion The detection of CEA mRNA, CK19 mRNA, TA and CEA in peripheral blood can be used to monitor the peripheral blood of lung cancer. CEA mRNA and TA are better than CK19 mRNA and CEA.