用免疫组化染色的方法，检测抗Ｋｉ－６７单克隆抗体ＭＩＢ－１在大肠腺瘤、腺瘤伴中～重度不典型增生及高、中、低分化腺癌中的表达。结果表明：ＭＩＢ－１的阳性表达，与大肠上皮性肿瘤的组织学分级、大小及转移和预后均呈正相关，对判断肿瘤的恶性度及估计预后有一定的参考价值。抗Ｋｉ－６７抗体ＭＩＢ－１是一个能有效反映福尔马林固定、石蜡包埋组织切片中细胞增殖活性的标记物 Immunohistochemical staining was used to detect the expression of anti-Ki-67 monoclonal antibody MIB-1 in large intestine adenomas, adenomas with moderate to severe dysplasia, and high, middle and poorly differentiated adenocarcinomas. The results showed that the positive expression of MIB-1 was positively correlated with the histological grade, size, metastasis and prognosis of epithelial tumors of the large intestine, and had a certain reference value for judging the malignancy of the tumor and estimating its prognosis. Anti-Ki-67 antibody MIB-1 is a marker that can effectively reflect cell proliferation activity in formalin-fixed, paraffin-embedded tissue sections