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目的 :探讨单层上皮细胞角蛋白 CK18和 CK19作为口腔癌前病变标志的可能性。方法 :用 L SAB免疫组化染色方法检测 CK18和 CK19在福尔马林固定、石蜡包埋的口腔正常粘膜、上皮单纯增生、轻度上皮异常增生、中度上皮异常增生、重度上皮异常增生和口腔鳞癌组织中的分布和表达强度 ,光镜观察染色切片 ,结果用秩和检验分析。结果 :正常和异常的口腔粘膜均未检测到 CK 18;CK 19可表达于正常非角化上皮的基底层细胞、异常增生上皮的基底上层细胞及口腔鳞状细胞癌中 ,阳性细胞数与上皮的异常增生程度及鳞癌的分化程度相关。结论 :CK19表达于口腔上皮基底上层细胞可作为诊断口腔癌前病变和判断口腔鳞癌分化程度的候选辅助指标之一。
Objective: To investigate the possibility of monolayer epithelial keratin CK18 and CK19 as markers of oral precancerous lesions. Methods: Immunohistochemical staining with L SAB was used to detect the expression of CK18 and CK19 in formalin-fixed, paraffin-embedded oral normal mucosa, epithelial hyperplasia, mild epithelial dysplasia, moderate epithelial dysplasia, severe epithelial dysplasia and Oral squamous cell carcinoma of the distribution and expression intensity, light microscopy staining sections, the results of rank sum test analysis. RESULTS: CK18 was not detected in both normal and abnormal oral mucosa. CK19 was expressed in basal lamina propria of normal non-keratinized epithelium, basal cells in dysplastic epithelium and oral squamous cell carcinoma, the number of positive cells and epithelium The degree of abnormal proliferation and the degree of differentiation of squamous cell carcinoma. Conclusion: The expression of CK19 in the basal epithelial cells of oral epithelium may be used as a candidate adjunct to diagnose oral precancerous lesions and to determine the differentiation of oral squamous cell carcinoma.