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内皮细胞(ECs)衬覆于血管内腔面,所构成的选择性生理屏障进行血管内外物质交换而保证了血管结构和功能的正常,ECs通过分泌多种生物活性因子来调节局部血管流场压力,以适应生理活动的变化。本研究采用改良流动培养的流室压力系统对ECs加载不同压力,同时设置静态培养、单纯剪应力对照组,结合ELISA法和定量RT-PCR技术,检测ECs内皮素-1(ET-1)、内皮型一氧化氮合成酶(eNOS)、环氧化酶-2(COX-2)及内皮细胞生长因子(VEGF)等功能蛋白分子的变化情况,从而观察流场内不同压力信号(特别是压力降信号)是否会引起ECs功能蛋白表达与合成异常,分析其量-效关系变化,以便进一步了解不同压力信号诱发内皮细胞功能障碍的机理。研究结果表明,ECs可以通过调节功能蛋白分子的表达来适应局部流场内压力信号的变化,而在流场压力降信号(-40 cmH2O)诱导的内皮细胞功能障碍过程中,eNOS、COX-2和VEGF在一定范围内起着保护ECs完整性的重要作用。
Endothelial cells (ECs) lining the vascular endothelium surface, the formation of a selective physiological barrier for the exchange of substances inside and outside the blood vessels to ensure the normal structure and function of blood vessels, ECs by regulating the secretion of a variety of biological activity of local vascular flow field pressure , To adapt to changes in physical activity. In this study, ECs were loaded with different pressures by flow chamber system with modified flow culture, and static culture and pure shear stress control group were set up. The levels of endothelin-1 (ET-1) in ECs were detected by ELISA and quantitative RT- (ENOS), cyclooxygenase-2 (COX-2), and endothelial cell growth factor (VEGF) were detected by flow cytometry in order to observe the effects of different pressure signals (especially pressure Down signal) could cause abnormal expression and synthesis of ECs functional protein, and analyze the relationship between quantity and effect, so as to further understand the mechanism of different pressure signals induced endothelial dysfunction. The results showed that ECs could adapt to the change of pressure signals in local flow field by regulating the expression of functional protein molecules. In the process of endothelial cell dysfunction induced by pressure drop signal of flow field (-40 cmH2O), the expression of eNOS, COX-2 And VEGF play an important role in protecting the integrity of ECs to a certain extent.