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采用免疫组织化学SABC法，分别对喉癌、声带息肉及正常声带组织的Bax基因进行检测。结果显示，Bax基因免疫组织阳性反应为棕黄色颗粒，表达部位位于细胞浆内。阳性表达分别为：95例喉癌中57例（60．0％），10例声带息肉中5例（50．0％），6例正常声带腺体中5例（83．3％）。喉癌吸烟患者与不吸烟患者的Bax基因表达阳性存在显著性差异（P＜0．05）。Bax基因表达阳性者3，5，10年生存率分别为86．6％，84．1％，79．9％；阴性者3，5，10年生存率分别为77．2％，72．5％，58．0％。Bax基因表达阳性者生存曲线明显高于其防性者；死亡危险度Bax基因表达阳性者波峰低于阴性者。提示Bax基因表达对喉癌的诊断与预后的判定具有重要的应用价值。
Immunohistochemical SABC method was used to detect the Bax gene in laryngeal carcinoma, vocal cord polyps and normal vocal cord tissues respectively. The results showed that Bax gene immunohistochemical positive brown particles, the expression site located in the cytoplasm. The positive expression was 57 cases (60.0%) in 95 cases of laryngeal cancer, 5 cases (50.0%) in 10 cases of vocal cord polyps and 5 cases (83.3%) in 6 cases of normal vocal cord glands. The positive expression of Bax gene in laryngeal cancer patients and non-smoking patients was significantly different (P <0.05). The 3, 5 and 10-year survival rates of Bax-positive patients were 86.6%, 84.1% and 79.9%, respectively. The 3, 5 and 10-year survival rates of negative patients were 77.2% and 72.5% %, 58.0%. The survival curve of patients with positive Bax gene expression was significantly higher than that of their prevention patients; the peak of death risk Bax gene expression was lower than those with negative. Tip Bax gene expression of laryngeal cancer diagnosis and prognosis of the decision has important application value.