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本文报道1986～1987年间从美国科罗拉多州和犹他州的20种水生鸟粪中分离到霍乱弧菌。取新鲜鸟粪、成年鸟和幼鸟肛拭子及河水样本分别接种在碱性蛋白胨水、TCBS琼脂平板以及94S4YL培养基上进行培养,取单个菌落做甘露糖、蔗糖和阿拉伯糖分解试验,并进行血清分型和Y-1肾上腺细胞毒力试验。结果:20种水生鸟的1131份粪及肛拭子中,约17.5％(198/1131)分离到霍乱弧菌。该菌的分离率与温度有关。当水温低于10℃时即分离不到霍乱弧菌。1986年12月至1987年1月,水及粪标本均分离不到霍乱弧
This article reports Vibrio cholerae isolated from 20 species of aquatic bird droppings in Colorado and Utah from 1986 to 1987. Fresh guano, adult birds and juvenile rectal swabs and river samples were inoculated on alkaline peptone water, TCBS agar plate and 94S4YL medium, respectively, and single colonies were used for mannose, sucrose and arabinose decomposition tests Serotyping and Y-1 Adrenal Cytotoxicity Assay were performed. Results: Vibrio cholera was isolated from about 17.5% (198/1131) of 1131 manure and anal swabs of 20 species of aquatic birds. The isolation rate of the bacteria and temperature. When the water temperature is below 10 ℃, Vibrio cholerae can not be isolated. From December 1986 to January 1987, cholera arcs could not be separated from water and fecal samples