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用四血管闭塞法造成大鼠一过性全脑缺血。按不同时程，以还原型尼克酰胺腺嘌呤二核苷酸脱氨酶组织化学方法对再灌流期间海马不同亚区内一氧化氮合酶阳性细胞的数量变化进行了研究。结果发现：海马本部的一氧化氮合酶阳性细胞数量在再灌流2～6h即有增高，到12～24h进一步增加至对照水平的3倍左右；3d时已有所减少，7d时在CA1区恢复至对照组的水平。齿状回的一氧化氮合酶阳性细胞数量在再灌流2～6h已有明显的增高，约为对照水平的2倍．并在再灌流12h、24h和3d时保持在同一水平。此外，缺血后各亚区内染出的血管数量于再灌流2～6h即有明显增多，并在再灌流7d后仍明显高于对照组。本文结合文献对缺血性神经元坏死和一氧化氮合酶表达之间的关系进行了讨论。
Transient global cerebral ischemia in rats caused by four-vessel occlusion. The changes of nitric oxide synthase positive cells in different subregions of hippocampus during reperfusion were studied by the reduction of nicotinamide adenine dinucleotide deaminase histochemical method at different time courses. The results showed that the number of nitric oxide synthase positive cells in the hippocampus was increased from 2 to 6 hours after reperfusion and further increased to 3 times of the control level from 12 to 24 hours; Recovery to the control group level. The number of nitric oxide synthase positive cells in dentate gyrus increased significantly from 2 to 6 hours after reperfusion, which was about 2 times of the control level. And remained at the same level at 12h, 24h and 3d after reperfusion. In addition, the numbers of blood vessels stained in each subregion after ischemia increased obviously from 2 to 6 hours after reperfusion and remained significantly higher than that of the control group 7 days after reperfusion. This article discusses the relationship between ischemic neuronal necrosis and nitric oxide synthase expression in the literature.