论文部分内容阅读
应用电镜及免疫电镜技术对 2 18例垂体腺瘤进行了观察分析 ;并根据瘤细胞超微结构及免疫细胞化学反应特点进行了分类。免疫电镜证实并非所有含纤维小体的瘤细胞为生长激素细胞 ,某些泌乳素细胞腺瘤亦可查见纤维小体 ,这提示纤维小体并不是垂体生长激素腺瘤特有的一个形态特点。本观察证实异位外排现象常属于少颗粒泌乳素细胞腺瘤的一个特点。促肾上腺皮质激素( ACTH)细胞腺瘤的分泌颗粒形状不规则 ,体积变异较大 ,瘤细胞内常含有成束的微纤维丝 ,有时瘤细胞核周围可形成环状纤维区。免疫电镜还揭示促性腺激素细胞腺瘤中含有卵泡刺激素细胞 ( FSH)或黄体生成素 ( L H)细胞 ,FSH细胞中可含有较多的分泌颗粒 ,体积小 ,电子密度高 ;L H细胞中分泌颗粒较多 ,体积大 ,形状可不规则 ,电子密度低。本研究对 2 1例无功能性垂体腺瘤进行了分析 ,提示这一组肿瘤可进一步分类为有功能腺瘤、嗜酸性干细胞瘤、大嗜酸细胞瘤及未分化细胞瘤。本文报道了这几类肿瘤的超微结构特点及免疫细胞化学反应特点
Electron microscopy and immunoelectron microscopy were used to observe and analyze 28 pituitary adenomas. The classification was based on the ultrastructure of tumor cells and the characteristics of immunocytochemical reaction. Immunoelectron microscopy confirmed that not all tumor cells containing fibrous bodies are growth hormone cells. Some prolactinomas can also be found in fibrous bodies, suggesting that fibrous bodies are not a characteristic feature of pituitary growth hormone adenomas. This observation confirms that the phenomenon of ectopic efflux is often a feature of the small-granular prolactinoma. Adrenocorticotropic hormone (ACTH) cell adenomas have irregular shape and large volume variation. The tumor cells often contain bundles of microfibrillar filaments, and sometimes the tumor cell nucleus can form circular fiber regions. Immunoelectron microscopy also revealed that gonadotropin cell adenomas contain follicle stimulating hormone (FSH) cells or luteinizing hormone (LH) cells. FSH cells may contain more secretory granules, which are small in size and high in electron density; they secrete in LH cells. More particles, large volume, irregular shape, low electron density. In this study, 21 cases of non-functional pituitary adenomas were analyzed, suggesting that this group of tumors can be further classified as functional adenoma, eosinophilic stem cell tumor, large eosinophiloma, and undifferentiated cell tumor. This article reports the ultrastructural characteristics and immunocytochemical reaction characteristics of these types of tumors.