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目的阐明2004年中国流行的甲3亚型流感病毒血凝素抗原性及其基因变异情况。方法对2004年分离的甲3亚型毒株先进行单向血凝抑制试验及交叉血凝抑制试验;在此基础上选取不同时间、地点的甲3亚型流感毒株进行血凝素基因HA1区核苷酸序列测定并推导出其氨基酸序列,然后进行基因进化特性分析。结果单向血凝抑制实验结果表明,2004年共有52.3%毒株与A/Fujian/411/2002(H3N2)(20042005毒株)有4倍或以上的血凝抑制滴度差异,交叉血凝抑制实验结果表明,它们间的抗原比为4。HA1区核苷酸序列和氨基酸序列分析表明,我国从2004年2月分离的甲3亚型毒株开始出现了与A/Fujian/411/2002(H3N2)和A/Wellington/1/2004(H3N2)(2005年国际代表株)相比较,在其HA1蛋白分子上存在有4个氨基酸位点(159位Y>F,189位S>N,145位K>N,226位V>I)发生了替换。此类毒株首发于我国南方,然后到我国北方。结论我国2004年2月份以后所分离的甲3亚型流感毒株已经发生抗原性及基因特性的改变。
Objective To elucidate the antigenicity and genetic variation of hemagglutinin of the Influenza A (3) virus in China in 2004. Methods Unidirectional hemagglutination inhibition test and cross hemagglutination inhibition test were carried out on the subtype A 3 isolates isolated in 2004. On the basis of this study, influenza A 3 strains of HA subtype HA1 Region of the nucleotide sequence determination and deduced amino acid sequence, and then analysis of gene evolution. Results The unidirectional hemagglutination inhibition test showed that there were 4 times or more of the titer of hemagglutination inhibition in 52.3% strains in 2004 and A / Fujian / 411/2002 (H3N2) (20042005 strain) Experimental results show that the antigen ratio between them is 4. The nucleotide and amino acid sequence analysis of HA1 showed that the isolates of A / Fujian / 411/2002 (H3N2) and A / Wellington / 1/2004 (H3N2 (159 Y> F, 189 S> N, 145 K> N, 226> V> I) on its HA1 protein molecule Replaced. Such strains first started in southern China, and then to the north of our country. Conclusion The antigenicity and gene characteristics of Influenza A (3) subtype influenza strains isolated in China after February 2004 have been changed.