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目的研究川芎嗪对成年大鼠局灶性脑缺血后神经元前体细胞迁移的影响,初步探讨川芎嗪对脑缺血损伤后功能恢复的作用。方法线栓法制作大鼠左侧大脑中动脉阻塞模型(MCAO),术后2h腹腔注射川芎嗪注射液(40mg/kg,每天1次)、分别于MCAO后3、7、14、21天采用免疫组织化学法观察神经元前体细胞的标志Doublecortin(DCX)在侧脑室室下区(SVZ)及SVZ吻侧迁移流(RMS)的迁移情况。结果缺血3天时,SVZ DCX阳性细胞经RMS迁移至嗅球,持续至21天;缺血7天时,SVZ直接及由RMS向邻近缺血纹状体迁移,14天时明显增加;21天时有少量DCX阳性细胞经胼胝体向缺血皮质迁移。川芎嗪组SVZDCX阳性细胞迁移途径同缺血模型组,但程度明显增强。结论川芎嗪可促进神经元前体细胞直接迁移至缺血皮质和纹状体,提示川芎嗪可能通过促进经元前体细胞的迁移对脑缺血后脑功能的自身恢复起重要作用。
Objective To study the effect of ligustrazine on neuronal precursor cell migration after focal cerebral ischemia in adult rats, and to explore the effects of ligustrazine on functional recovery after cerebral ischemic injury. METHODS: The rat left middle cerebral artery occlusion model (MCAO) was made by a line embolization method. Ligustrazine injection (40 mg/kg, once daily) was given intraperitoneally 2 hours after the operation, and was used 3, 7, 14, 21 days after MCAO. Immunohistochemistry was used to observe the migration of neuronal precursor cells, Doublecortin (DCX), in the lateral ventricle subventricular zone (SVZ) and the SVZ kiss-side migration (RMS). RESULTS: At 3 days of ischemia, SVZ DCX-positive cells migrated to the olfactory bulb through RMS and lasted to 21 days; at 7 days of ischemia, SVZ migrated directly and from RMS to adjacent ischemic striatum and increased significantly at 14 days; there was a small amount of DCX at 21 days. Positive cells migrated from the corpus callosum to the ischemic cortex. Ligustrazine group SVZDCX positive cell migration pathway is the same as the ischemia model group, but the degree is significantly enhanced. Conclusion Ligustrazine can promote the direct migration of neuronal precursor cells to the ischemic cortex and striatum, suggesting that tetramethylpyrazine may play an important role in the recovery of brain function after cerebral ischemia by promoting the migration of mesenchymal precursor cells.