报道了转基因抗虫水稻克螟稻 1号 (Ts 9)与非转基因恢复系川恢 94 9、H97810 17及保持系 2 12B等杂交F3 代的GUS和PCR检测。GUS检测的结果 ,Ts 9与非转基因川恢 94 9、H97810 17杂交F3 代有较多的GUS阳性植株。GUS阴性植株与阳性植株的分离比约为 2∶1。用设计合成的一对引物进行PCR扩增的结果 ,大部分GUS检测呈阳性的植株及一个保持系 2 12B与Ts 9杂交F3 代植株获得了大小约 12 0 0bp的PCR产物 ,即cryIA (b)基因片段。试验结果表明PCR是比GUS叶片染色法更为灵敏、准确、方便的检测方法。 The GUS and PCR assays were reported for the crosses between transgenic insect-resistant rice Ketiodao 1 (Ts 9) and non-transgenic restorer lines Chuanhui 94 9, H97810 17 and maintainer line 2 12B. GUS test results, Ts 9 and non-transgenic Sichuan Hui 94 9, H97810 17 cross F3 generations have more GUS-positive plants. The isolation ratio of GUS-negative plants to positive plants is about 2: 1. A pair of primers designed for PCR amplification results, most of the GUS test positive plants and a maintainer line 12 12 and Ts 9 cross F3 plants obtained a size of about 1200bp PCR products, namely cryIA (b )Gene fragment. The test results show that PCR is more sensitive, accurate and convenient than the GUS leaf staining method.