目前,细胞培养技术已广泛应用于实验室,培养要求辅加的动物血清较普通的是胎牛血清或新生牛血清。牛血清来源困难,价格昂贵,而且市售的血清质量也不稳定。本室对细胞培养液的辅加成份进行了实验研究,结果证明猪血清、胎浆、稀土等在支持细胞培养和增殖方面是可以起到辅助或替代作用的。 材 料 与 方 法 一、胎浆3～4个月水囊引产的胎儿,生理盐水洗涤,加等体积Hank’s液匀浆,-60℃冻溶后,离心6000r/min,30min,收取上清过滤,56℃30min灭活。 二、氯化释土(RECl_3·6H_2O)MW At present, the cell culture technology has been widely used in laboratories, training additional animal serum culture is more common is fetal bovine serum or neonatal bovine serum. Bovine serum sources are difficult, expensive, and commercially available serum quality is not stable. The chamber of the cell culture fluid supplemented by ingredients of the experimental study, the results prove that pig serum, fetal plasma, rare earth and other support cell culture and proliferation can play an auxiliary or alternative role. Materials and methods First, the fetus 3 to 4 months after induction of labor, saline washing, plus equal volume of Hank’s liquid homogenate, frozen at -60 ℃, centrifuged 6000r / min, 30min, supernatant filtration, 56 ℃ 30min inactivation. Second, the release of chloride (RECl_3 · 6H_2O) MW