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目的:建立用于研究下颌骨骨缺损基因治疗的实验动物模型及基因导入方法。方法:用30只新西兰大白兔作为实验动物,在左侧下颌骨体部造成直径5mm圆形贯通性骨缺损,以明胶海绵作为载体,将含有人肝细胞生长因子全长cDNA片段的表达型质粒(pUDKH)直接应用于15只兔骨缺损的局部,对另外15只应用等量空质粒(pUDK)作为对照,对骨缺损的愈合情况进行大体形态学、X线和组织学评价。结果:下颌骨骨缺损修复过程中,在形态学、X线和组织学等方面,应用pUDKH组的骨愈合情况优于pUDK组。结论:此实验建立的下颌骨骨缺损实验动物模型,以及通过直接应用细胞因子裸DNA治疗下颌骨骨缺损的基因导入途径,为骨再生和骨缺损的基因治疗研究提供了新的方法。
OBJECTIVE: To establish an experimental animal model and gene introduction method for studying gene therapy of mandibular defects. Methods: Thirty New Zealand white rabbits were used as experimental animals, and 5 mm in diameter and 5 mm in diameter were formed on the left mandibular body. Gelatin sponge was used as the carrier. The expression plasmids containing the full-length cDNA of human hepatocyte growth factor (pUDKH) was applied directly to the top of 15 rabbit bone defects. The other 15 rabbits were subjected to general morphology, X-ray and histological evaluation using pUDK as a control. Results: The bone healing of pUDKH group was better than that of pUDK group in terms of morphology, X-ray and histology in the process of mandibular defect repair. CONCLUSION: The animal model of mandibular defects established by this experiment and the introduction of genes for the treatment of mandibular defects by direct application of naked cytokines provide a new method for the gene therapy of bone regeneration and bone defects.