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AIM:To study the inhibitory effects of antisense RNA ofHAb18G/CD147 on invasion of hepatocellular carcinoma(HCC)cells in vitro.METHODS:Antisense RNA of HAb18G/CD147 vector PCI-asHAb18G was constructed by reversely inserting HAb18G/CD147 cDNA to eukaryotic expression vector PCI-neo.TheHCC cell line HHCC was transfected by PCI-asHAb18G viacation liposome.Expression of HAb18G/CD147 of transfectedcells selected by G418(geneticin)was observed by immuno-histochemical SP staining and FACS(fluorescence activatedcell sorting).Gelatin zymography was used to determinethe effect of PCI-asHAb18G on reducing secretions of MMP-2 and MMP-9 of the transfected cells.Boyden chamber wasemployed to test the invasion of HCC cells in vitro.RESULTS:The construction of antisense RNA vector PCI-asHAb18G was verified correct by partial nucleotidesequencing and restricted endonuclease digestion.Theexpression of HAb18G/CD147 in transfected HHCC wasinhibited by PCI-asHAb18G.Secretions of MMP-2 and MMP-9 of transfected HHCC were reduced and the invasion oftransfected HHCC was inhibited compared to HHCC,respectively.CONCLUSION:Invasion of HCC cells can be inhibited byantisense RNA of HAb18G/CD147.HAb18G/CD147 may beused as a potential target of drugs for anti-invasion andmetastasis of HCC.
AIM: To study the inhibitory effects of antisense RNA of HAb18G / CD147 on invasion of hepatocellular carcinoma (HCC) cells in vitro. METHODS: Antisense RNA of HAb18G / CD147 vector PCI-asHAb18G was constructed by reversely inserting HAb18G / CD147 cDNA to eukaryotic expression vector PCI-neo. The HCC cell line HHCC was transfected by PCI-as HAb18G viacation liposome. Expression of HAb18G / CD147 of transfected cells selected by G418 (geneticin) was observed by immuno-histochemical SP staining and FACS (fluorescence activated cell sorting). Gelatin zymography was used The determination of the effect of PCI-as HAb18G on reducing secretions of MMP-2 and MMP-9 of the transfected cells. Boyden chamber wasemployed to test the invasion of HCC cells in vitro .RESULTS: The construction of antisense RNA vector PCI-asHAb18G was verified correct by partial nucleotide sequencing and restricted endonuclease digestion. The expression of HAb18G / CD147 in transfected HHCC was inhibited by PCI-as HAbl8G. Secretions of MMP-2 and MMP-9 of transfec Ted HHCC were reduced and the invasion of transfected HHCC was inhibited compared to HHCC, respectively. CONCLUSION: Invasion of HCC cells can be inhibited byantisense RNA of HAb18G / CD147.HAb18G / CD147 may be used as a potential target of drugs for anti-invasion and metastasis of HCC.