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用两种β-hCG抗血清SB-6和LB-85,建立了2小时快速放射免疫法,灵敏度为10mIU/ml,NBS<5%,批内测定误差<11%,批间误差<15%,临床准确性和特异性都为100%。37℃抗原抗体温育2小时达到平衡状态。调查了61例新婚妇女血清hCG水平,于月经周期22～29天诊断出其中14人已妊娠,hCG水平大于10mIU/ml;另47例未孕,hCG水平低于10mIU/ml。最早诊断时间为排卵后第8天。用两种方法,快速法和标准法(24小时)同时测定61例新婚妇女,43例闭经妇女和107例葡萄胎患者血清hCG水平,结果表明两种方法有十分显著相关性,r=0.94,p<0.001,y=19.55+0.93x。
Two-hour rapid radioimmunoassay was performed using two β-hCG antiserums, SB-6 and LB-85, with a sensitivity of 10 mIU / ml, NBS <5%, intra- assay error <11%, batch error <15% The clinical accuracy and specificity were both 100%. 37 ℃ antigen antibody incubated for 2 hours to reach equilibrium. 61 cases of newlywed women were investigated serum hCG levels, 22 to 29 days in the menstrual cycle diagnosis of which 14 were pregnant, hCG levels greater than 10mIU / ml; the other 47 cases of non-pregnancy, hCG levels below 10mIU / ml. The earliest diagnosis time was 8 days after ovulation. The serum hCG levels in 61 newly married women, 43 amenorrhea women and 107 hydatidiform mole patients were measured simultaneously by two methods, rapid method and standard method (24 hours). The results showed that there was a significant correlation between the two methods (r = 0.94, p <0.001, y = 19.55 + 0.93x.