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应用分子杂交及免疫组化方法检测５６例ＨＣＣ组织内的抑癌基因ｐ１６。结果显示，ＨＣＣ标本中ｐ１６蛋白在癌细胞内表达的阳性率为３７５％（２１／５６），而ｐ１６ＤＮＡ斑点杂交的阳性率为５５３６％（３１／５６）。Ｓｏｕｔｈｅｒｎ转膜杂交证实，１２５％（７／５６）ＨＣＣ标本存在ｐ１６的甲基化变异，４４６４％（２５／５６）ｐ１６基因缺失。提示ＨＣＣ发生、发展过程中存在ｐ１６基因的缺失和甲基化变异
The tumor suppressor gene p16 in 56 HCC tissues was detected by molecular hybridization and immunohistochemistry. The results showed that the positive rate of p16 protein expression in cancer cells was 37.5% (21/56) in HCC specimens, and the positive rate of p16 DNA dot blot hybridization was 55.6% (31/56). Southern membrane hybridization confirmed that there was a methylation mutation of p16 in 12 5% (7/56) HCC samples, and 44.64% (25/56) p16 gene deletion. It is suggested that there is deletion of p16 gene and methylation mutation during the development and progression of HCC.