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目的探讨脑血管病与MTHFR基因A1298C多态性关系。方法对我院脑血管病患者与健康体检者各100例作病例-对照研究。运用聚合酶连反应-限制性内切酶片段长度多态性分析(PCR-RFLP)方法确定MTHFR基因型。结果患者组中CC型与T等位基因频率、AC型与C等位基因频率均明显高于健康对照组(P<0.05)。结论 MTHFR1298AC型与C等位基因在脑血管疾病中的构成比均明显增大,MTHFR1298C多态性可能与脑血管疾病的发生存在一定的相关性。
Objective To investigate the relationship between cerebrovascular disease and MTHFR gene A1298C polymorphism. Methods A case-control study of 100 cases of cerebrovascular patients and healthy subjects in our hospital. The MTHFR genotype was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results The frequency of CC type and T allele and the frequency of AC type and C allele were significantly higher in patients than in healthy controls (P <0.05). Conclusion The proportions of MTHFR1298AC and C allele in cerebrovascular disease are significantly increased. The polymorphism of MTHFR1298C may be related to the occurrence of cerebrovascular disease.