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目的研究人卵巢组织中乙型肝炎病毒表面抗原(HBsAg)和HBV DNA的表达与垂直传递的相关性。方法对33例血清HBsAg和HBeAg均阳性的孕妇,采用免疫组化SP法检测卵巢组织中HBsAg,荧光定量PCR法检测卵巢组织中HBV DNA的表达。分析HBsAg和HBV DNA的表达与垂直传递的相关性。结果 33例孕妇中,7例卵巢组织HBsAg阳性,婴儿宫内感染率42.85%(3/7);其中的2例在卵泡中呈阳性表达,卵泡阳性的宫内感染率100%(2/2)。卵巢组织HBV DNA阳性12例(HBV DNA定量>1.0×106copies/ml),婴儿宫内感染率33.33%(4/12)。宫内感染婴儿较非宫内感染婴儿母亲卵巢组织中HBV DNA的表达水平和阳性率均明显升高,但差异无统计学意义。结论 HBV可感染人卵巢组织,且有可能通过卵细胞垂直传递至子代。
Objective To study the correlation between the expression of hepatitis B virus surface antigen (HBsAg) and HBV DNA in human ovarian tissue and the vertical transmission. Methods Totally 33 pregnant women with positive serum HBsAg and HBeAg were tested for HBsAg in ovarian tissue by immunohistochemical SP method and HBV DNA expression in ovarian tissue by fluorescence quantitative PCR. The correlation between HBsAg and HBV DNA expression and vertical transmission was analyzed. Results Of the 33 pregnant women, 7 cases had positive HBsAg in ovary tissues and the intrauterine infection rate was 42.85% (3/7). Two of them were positive in the follicles and the rate of intrauterine infection in the follicles was 100% (2/2 ). In ovarian tissue, 12 cases were positive for HBV DNA (HBV DNA> 1.0 × 106 copies / ml), and the intrauterine infection rate in infants was 33.33% (4/12). The expression level and positive rate of HBV DNA in intrauterine infection infants were significantly higher than those in non-intrauterine infection infants’ mothers, but the difference was not statistically significant. Conclusions HBV can infect human ovarian tissue and may pass vertically to the offspring through egg cells.