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目的：探讨Ｂ７－１基因修饰的肿瘤细胞作为瘤苗对小鼠胃癌的治疗效果。方法：在已建立高表达Ｂ７－１分子的小鼠前胃癌细胞系ＭＦＣ－Ｂ７的基础上，观察ＭＦＣ－Ｂ７细胞体外激活脾淋巴细胞的能力，体内成瘤性变化及作为瘤苗对荷瘤鼠胃癌的治疗效果。结果：ＭＦＣ－Ｂ７细胞在体外能有效诱导脾淋巴细胞的活化增殖和特异性ＣＴＬ（ＣｙｔｏｔｏｘｉｃＴｌｙｍｐｈｏｃｙｔｅｓ）活性的产生，在体内的成瘤能力明显下降，作为瘤苗接种对荷瘤鼠胃癌有较好的治疗效果。结论：Ｂ７－１基因修饰的肿瘤细胞免疫原性明显增强，有希望作为瘤苗用于此类肿瘤的治疗。
Objective: To investigate the effect of B7-1 gene-modified tumor cells as tumor vaccines on gastric cancer in mice. METHODS: On the basis of the establishment of a mouse gastric cancer cell line MFC-B7 with high expression of B7-1 molecule, the ability of MFC-B7 cells to activate splenic lymphocytes in vitro was investigated. The tumorigenicity in vivo was changed and used as a tumor vaccine to tumor-bearing tumors. Rat gastric cancer treatment effect. RESULTS: MFC-B7 cells could effectively induce the proliferation of spleen lymphocytes and the production of specific CTL (CytotoxicTlymphocytes) activity in vitro. The tumorigenicity in vivo was significantly decreased. treatment effect. Conclusion: The immunogenicity of B7-1 gene-modified tumor cells was significantly enhanced, and it is hopeful that these tumors can be used as tumor vaccines.