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以限制性内切酶（ＨｐａⅡ）消化ＤＮＡ，应用ＰＣＲ方法敏感检测７５例恶性血液病病人降钙素基因的甲基化程度和１０例正常对照。结果：１０例正常对照均系正常甲基化；８１％急性白血病、８８％ＭＤＳ、８０％ＮＨＬ的病人出现降钙素基因高甲基化阳性；再障病人为正常甲基化。ＣＭＬ病人降钙素基因甲基化程度与病期有密切关系，慢性期大多为正常甲基化，加速期和急变期表现为降钙素基因高甲基化阳性。这一结果提示：降钙素基因高甲基化可作为恶性血液病克隆性增殖的分子基因标志；可作为微小残留病（ＭＲＤ）检测的手段。
DNA was digested with restriction enzyme (HpaII) and the degree of methylation of calcitonin gene in 75 patients with hematologic malignancy was detected by PCR and 10 normal controls. Results: 10 normal controls were all normal methylation; 81% of patients with acute leukemia, 88% of MDS, and 80% of NHL had positive hypermethylation of calcitonin gene; patients with aplastic anemia were normal methylation. The degree of methylation of calcitonin gene in patients with CML is closely related to the stage of disease. Most of them are normal methylation in the chronic phase and hypermethylation of the calcitonin gene is positive in the accelerated phase and blast phase. This result suggests that the hypermethylation of calcitonin gene can be used as a molecular marker for the clonal proliferation of hematological malignancies; it can be used as a means for detection of minimal residual disease (MRD).