在研制疟疾疫苗的过程中,子孢子受到了特别的重视。蚊叮咬健康人时,子孢子进入人肝脏。有效的抗子孢子虫苗应使预防接种者不发病,从而阻断疟疾的传播。这方面的最大困难是不易得到足够研制疫苗用的子孢子。来自蚊唾腺的子孢子远远不能满足成千上万人预防接种的需要,亦不能适应基因克隆化及合成抗原蛋白的需要。“经典”的克隆化方法是先要得到与所需 Sporozoites have received special attention during the development of the malaria vaccine. When mosquitoes bite healthy people, sporozoites enter the human liver. Effective anti-sporozoa vaccine should prevent vaccinates from getting sick and thus prevent the spread of malaria. The biggest difficulty in this area is that it is not easy to get enough sporozoites for vaccines. Sporozoites from mosquito salivary glands are far from meeting the needs of thousands of people for vaccination and can not meet the needs of gene cloning and the synthesis of antigenic proteins. The “classic” method of cloning is to first get and what is needed