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目的研究miR-137过表达对人胃癌MKN-45细胞迁移侵袭的影响。方法采用RT-PCR法检测5株胃癌细胞和人胃黏膜上皮细胞(GES)中miR-137的表达。构建miR-137慢病毒,并转染MKN-45细胞。通过划痕实验、细胞侵袭小室检测和Transwell侵袭实验以分析过表达miR-137对MKN-45细胞迁移侵袭的影响。结果 MKN-45细胞中miR-137的表达丰度明显低于在GES、MKN-74、AGS、SGC-7901和BGC-823细胞,差异有统计学意义(P<0.05)。同时成功构建micro up病毒感染的细胞组。划痕实验结果显示,与空白对照组(CON组)和阴性对照组(NC组)比较,micro up组24、48 h后平均迁移率差异有统计学意义(P<0.05);细胞侵袭小室检测结果显示,与CON组、NC组比较,micro up组的平均迁移率差异有统计学意义(P<0.01);Transwell侵袭实验结果显示,与CON组、NC组比较,micro up组平均迁移率差异有统计学意义(P<0.01)。结论 miR-137过表达能明显抑制人胃癌MKN-45细胞迁移侵袭能力,有可能成为胃癌治疗的新靶点。
Objective To investigate the effect of miR-137 overexpression on the migration and invasion of human gastric cancer cell line MKN-45. Methods The expression of miR-137 in 5 gastric cancer cells and human gastric epithelial cells (GES) was detected by RT-PCR. MiR-137 lentivirus was constructed and transfected into MKN-45 cells. Scratch assay, cell invasion chamber assay and Transwell invasion assay were used to analyze the effect of miR-137 overexpression on MKN-45 cell migration and invasion. Results The abundance of miR-137 in MKN-45 cells was significantly lower than that in MKN-74, AGS, SGC-7901 and BGC-823 cells (P <0.05). At the same time, micro cell-infected cells were successfully constructed. Scratch test results showed that, compared with the blank control group (CON group) and the negative control group (NC group), the micro-group 24 and 48 h after the average mobility was significantly different (P <0.05); cell invasion chamber detection The results showed that compared with CON group and NC group, the average migration rate of micro up group was statistically significant (P <0.01). The results of Transwell invasion assay showed that the average migration rate of micro up group was significantly higher than that of CON group and NC group There was statistical significance (P <0.01). Conclusion Overexpression of miR-137 can significantly inhibit the migration and invasion of human gastric cancer MKN-45 cells and may be a new therapeutic target for gastric cancer.