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目的探讨瘦素(leptin)在糖尿病肾脏病(DN)变发生发展中的作用。方法雄性Wistar大鼠70只,随机分为实验组和对照组,实验组40只,喂以高热量饲料2个月后,腹腔注射链脲佐菌素(STZ)30mg/kg;对照组30只,喂以普通饲料2个月后,腹腔注射等体积的枸橼酸缓冲液。两组分别于注射前、注射后1、2、3和6个月各处死6只,取血测定血清瘦素、胰岛素水平等,光镜电镜观察肾脏形态学改变。结果成模后1月后肾小球体积开始增大,2月更明显,3月及6月体积显著下降;成模后2月出现明显蛋白尿,肾小球基底膜3月时就已显著增厚,6月时更为严重;瘦素水平在高热量饮食2月后即较对照组显著增高(P<0.01),在注射STZ后1个月时由于体重的下降而较前有所下降,但仍高于对照组(P<0.01),以后随着病程的进展呈逐渐升高趋势。结论进一步证明瘦素可能是促进糖尿病肾脏病变的发生、发展的因素之一。
Objective To investigate the role of leptin in the development of diabetic nephropathy (DN). Methods Seventy male Wistar rats were randomly divided into experimental group and control group, 40 rats in experimental group were fed with high-calorie feed for 2 months and 30 mg / kg streptozotocin (STZ) , Fed with normal feed 2 months after intraperitoneal injection of an equal volume of citrate buffer. Six rabbits were sacrificed at 1, 2, 3, and 6 months after injection. Serum leptin and insulin levels were determined by blood samples. Morphological changes of the kidneys were observed under light microscope. Results The glomerular volume began to increase after 1 month and became more obvious in February, with a significant decrease in volume in March and June. Significant proteinuria occurred in February after modeling, and glomerular basement membrane was significant at 3 months Thickened and worsened in June. Leptin level was significantly higher in the high-calorie diet group than in the control group (P <0.01) after 2 months of high-calorie diet, but decreased slightly compared with the previous one after the STZ injection , But still higher than that of the control group (P <0.01), then gradually increased with the progress of the disease. Conclusions Leptin is further evidence that it may be one of the factors to promote the development of diabetic nephropathy.