论文部分内容阅读
目的:将培养扩增的猪膀胱移行上皮细胞接种到自制的膀胱脱细胞基质(BAM)片上,探讨此法体外构建用于新膀胱黏膜移行上皮化改造的组织工程复合物的可行性。方法:取新鲜猪膀胱制备BAM片,将原代猪膀胱移行上皮细胞进行体外培养、扩增后接种到BAM片上,培养成多层细胞结构的组织工程复合物。通过组织学观察,了解所制备的BAM结构及细胞在BAM片支架上的黏附及生长情况。结果:此法制备的BAM片具有较完整的胶原结构。细胞在BAM片上黏附、生长和增殖良好,可形成多层细胞的组织工程复合物。结论:该方法可成功培养猪膀胱移行上皮细胞。制备的BAM片生物相容性良好。膀胱移行上皮细胞与BAM片联合培养构建成具有多层细胞的组织工程膀胱黏膜替代物,有望用于新膀胱的移行上皮重建。
OBJECTIVE: To inoculate the cultured porcine bladder transitional epithelial cells into a homemade bladder acellular matrix (BAM) piece, and to explore the feasibility of this method in vitro to construct a tissue engineering complex for the transitional neoplasia of the new bladder mucosa. Methods: Fresh pig bladder was used to prepare BAM tablets. The primary porcine bladder transitional epithelial cells were cultured in vitro. After expansion, they were inoculated into BAM slices and cultured into multilayered tissue engineering composite. Through histological observation, to understand the prepared BAM structure and cells in the BAM stent scaffold adhesion and growth. Results: BAM tablets prepared by this method have a more complete collagen structure. Cells adhere to BAM and grow and proliferate well, forming multilayered tissue engineering complexes. Conclusion: This method can successfully culture pig bladder transitional epithelial cells. The prepared BAM tablets have good biocompatibility. Bladder transitional epithelial cells and BAM film co-culture into a multi-layer cell tissue engineering bladder mucosal substitute, is expected to be used for the reconstruction of the new bladder transitional epithelium.