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目的观察丙型肝炎病毒3种标志物的稳定性。方法用HCV-Ab、RT-PCR及HCV-Ag检测试剂分别检测血清样品中的HCV-Ab、HCV-RNA和HCV-Ag,比较3种标志物在不同温度、不同时间下保存及冻融后的稳定性。结果在2036份血清样品中,共检出HCV-Ab阳性样品85份、HCV-Ag阳性样品51份,HCV-RNA阳性样品12份;HCV-Ab4℃保存14d、-20℃保存3年及冻融5次稳定性良好,阳性率均为100%;HCV-RNA随保存时间的延长,阳性检出率逐步下降,-20℃保存3年后下降至33.33%,冻融后下降至83.33%;HCV-Ag-20℃保存3年后,阳性率下降至68.63%,4℃保存14d和冻融对其无影响。结论3种标志物中,HCV-Ab的稳定性最好,其次是HCV-Ag,HCV-RNA最不稳定。
Objective To observe the stability of three markers of hepatitis C virus. Methods HCV-Ab, HCV-RNA and HCV-Ag in serum samples were detected by using HCV-Ab, RT-PCR and HCV-Ag detection reagents. The three markers were stored at different temperatures and different times, Stability. Results In 2036 serum samples, 85 HCV-Ab positive samples, 51 HCV-Ag positive samples and 12 HCV-RNA positive samples were detected. HCV-Ab was stored at 4 ℃ for 4 days and stored at -20 ℃ for 3 years and frozen The positive rate of HCV-RNA decreased gradually after storage for 5 years and then decreased to 33.33% after 3 years of storage at -20 ° C and then decreased to 83.33% after freezing and thawing. After three years of storage at 20 ℃, the positive rate of HCV-Ag-20 was reduced to 68.63%. After stored at 4 ℃ for 14 days, freezing-thawing had no effect on HCV-Ag-3. Conclusion Among the three markers, HCV-Ab has the best stability, followed by HCV-Ag, and HCV-RNA is the most unstable.