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云南烟草丛枝症病害病程表现为潜伏、过敏反应、缩顶、丛枝等4个阶段,以过敏反应导致叶片严重坏死和枯死为前期特征,以腋芽丛生而表现为黄化丛枝症为后期特征,苗期感染形成僵苗症,大田期感染表现丛枝症;汁液摩擦接种11科32种植物发现其只侵染烟属植物,通过机械传染(嫁接、汁液)和介体传染(菟丝子、蚜虫、烟盲蝽、叶蝉)等方式传播,该病害与文献记载的烟草丛枝、丛顶和丛蔟类病害有明显区别,可能是未见记载的病害.在云南烟草丛枝症病株的老叶和带毒蚜虫中抽提到直径约22~24nm的球状病毒,回接烟草发病,病株检测到和云南烟草丛枝症病害相关的低分子量RNA,说明该病毒和云南烟草丛枝症病害有密切的关系,且与SBMV,VTMV,TRSV,ArMV,CarMV,CMV,BCMV,TNV等8种病毒无血清学关系,综合各项研究和文献检索结果,作者推测该病毒可能是未见记载的病毒,建议称为“烟草簇矮病毒(Tobacobushydwarfvirus)”.
The disease course of tobacco witches’ bush disease in Yunnan showed four stages: latent, anaphylactoid reaction, contracted top, and twig. With the allergic reaction leading to the serious necrosis and withering of the leaves, the axillary buds clustered with axillary buds as the later characteristics, Seedlings infected with the formation of a solidified seedling disease, field performance during the field withered twig; juice frictional inoculation of 32 species of plants found that they only infected tobacco plants, through mechanical infection (grafting, juice) and mediators (dodder, aphids, Tobacco planthopper, leafhopper) and other means of transmission, the disease and documented tobacco bush, Cong Cong and Cong Cong disease is significantly different, may be undocumented disease. In the old leaves and infected aphids of tobacco witches broom disease in Yunnan province, a spherical virus with a diameter of about 22-24 nm was extracted and the tobacco was introduced back to the diseased plants. Low molecular weight RNAs associated with the disease of Yunnan tobacco witches’ bush were detected in diseased plants, The results showed that there was a close relationship between the virus and Culex pipiens pallens disease and there was no serological relationship with eight viruses such as SBMV, VTMV, TRSV, ArMV, CarMV, CMV, BCMV and TNV. Based on the results of various studies and literature search, The authors speculate that this virus may be an undocumented virus, which is suggested as “Tobacobushydwarfvirus”.