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目的观察N-乙酰半胱氨酸(NAC)干预支气管哮喘模型后,其肺脏树突状细胞(DC)表面分子表达的变化及其对T淋巴细胞分化的影响。方法卵蛋白(OVA)致敏、激发裸鼠(BALB/C)哮喘模型后随机分成对照(C)组、地塞米松干预(B)组和NAC干预(A)组,每组16只。ELISA法进行检测血清总IgE、肺泡灌洗液(BLAF)和肺脏DC和DO11.10(OVA特异性转基因小鼠)脾脏T淋巴细胞共培养液上清中白细胞介素(IL)13和γ干扰素(IFN-γ)水平。结果A、B组血清IgE水平低于C组(P<0.01)。A、B组BALF中IL-13低于C组,而IFNγ-水平升高于C组(P<0.01)。A、B组肺脏DC表面分子CD80、CD86、MHCⅡ下调。与C组比较,小鼠肺脏DC和T淋巴细胞共培养后,A、B组上清IL-13水平明显降低,IFN-γ水平明显升高(P<0.01)。结论NAC可以抑制哮喘免疫应答反应向Th2方向偏移,且它的治疗作用可能是通过肺脏DC来实现的。
Objective To observe the changes of the molecular expression of dendritic cells (DCs) on the surface of lung and the effect on the differentiation of T lymphocytes after the intervention of NAC (acetylcysteine) in bronchial asthma model. Methods BALB / C asthma model was induced by OVA sensitization. The rats were randomly divided into control group (C), dexamethasone intervention group (B) and NAC intervention group (A), 16 rats in each group. ELISA was used to detect interleukin (IL) 13 and gamma in supernatant of splenic T lymphocyte from serum total IgE, alveolar lavage fluid (BLAF) and lung DC and DO11.10 (OVA specific transgenic mice) Superoxide dismutase (IFN-γ) levels. Results The level of serum IgE in groups A and B was lower than that in group C (P <0.01). The levels of IL-13 in BALF in group A and B were lower than those in group C, while the level of IFN-γ in BALF was increased in group C (P <0.01). A, B group of lung DC surface molecules CD80, CD86, MHC Ⅱ down regulation. Compared with group C, after co-cultured with DCs and T lymphocytes, the levels of IL-13 in supernatants of group A and group B were significantly decreased and the level of IFN-γ was significantly increased (P <0.01). Conclusion NAC can inhibit the shift of asthma immune response towards Th2, and its therapeutic effect may be achieved through pulmonary DC.