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目的探讨单核细胞增生李斯特菌多位点序列分型(MLST)与耐药性的关联性,确定某些具有高致病性潜能的流行克隆株的存在。方法采用Kirby-Bauer(K-B)纸片扩散法和E-test药敏试条法对14种抗生素进行药物敏感性试验,以MLST技术对50株菌株进行基因分型。结果单核细胞增生李斯特菌耐药率为22.00%(11/50),并出现多重耐药株。50株单核细胞增生李斯特菌MLST分析共获得12个型别,以ST9和ST121为优势型别。结论特定ST型别在食品生产过程中存在特定菌株之间的传递,人源性和食源性单核细胞增生李斯特菌中均发现耐药株,可能存在耐药基因的传递,应加强对具有潜在致病性的ST型别的监测力度。
Objective To investigate the association between multilocus sequence typing (MLST) and drug resistance of Listeria monocytogenes and to determine the existence of some popular clonal strains with high pathogenic potential. Methods Fourteen antibiotics were tested for drug susceptibility using Kirby-Bauer (K-B) disk diffusion method and E-test method. Fifty strains were genotyped by MLST. Results The resistance rate of Listeria monocytogenes was 22.00% (11/50), and multi-drug resistant strains appeared. Fifty strains of Listeria monocytogenes were analyzed by MLST, and 12 genotypes were obtained. ST9 and ST121 were dominant genotypes. CONCLUSIONS: Specific strains of ST exist in the process of food production. The resistant strains are found in both human and food-borne Listeria monocytogenes and there may be resistance gene transfer. Therefore, Potentially pathogenic ST-type surveillance efforts.