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本文介绍了一种贮存锡兰勾虫幼虫的脱鞘冰冻方法。采用该法保存一年的幼虫仍具有感染性,为研究工作提供了一种长期保存感染期幼虫的方法。实验用的感染期幼虫是用锡兰勾虫卵阳性的犬粪和消毒土一起培养出来的。操作方法:先用0.05%氯化钠溶液把分离到的幼虫洗2次,再用二氧化碳使其脱鞘,然后把幼虫放到具有螺旋盖的玻瓶内沉淀,尽可能吸去氯化钠溶液,旋紧瓶盖,将瓶置于一种生物
This article describes a method of sheath de-icing and freezing of tick-borne larvae in Ceylon. The larvae preserved for one year by this method are still infective and provide a method for the long-term preservation of infection larvae in research. The experimental infection larvae were cultured with cephalothorax-positive canine faeces and sterilized soil. Method of operation: first with 0.05% sodium chloride solution of the larvae were washed twice, and then use carbon dioxide to destruct, and then the larvae placed in a glass bottle with a screw cap precipitation, try to suck sodium chloride solution , Tighten the cap, the bottle placed in a living creature