论文部分内容阅读
目的:观察茯苓多糖对卵清白蛋白(OVA)诱导的小鼠肠道黏膜免疫应答的影响,探讨其对黏膜免疫的调节作用及其相关机制。方法:BALB/c小鼠随机分为2组:对照组和茯苓多糖组,对照组口服给予等体积生理盐水,茯苓多糖组每日按体重口服给药1次,剂量为200 mg·kg-1,2组小鼠给药1周后,1次性给予每只小鼠口服抗原卵清白蛋白(OVA)5 mg进行免疫,分别于免疫后1,2,3周,收集小鼠粪便样本,ELISA检测小鼠粪便OVA特异性分泌型免疫球蛋白A(sIgA);2组小鼠给药1周后,分离小鼠派氏结,流式细胞术检测派氏结(Peyer’s patches)中B淋巴细胞共刺激分子CD80,CD86表达。结果:对照组小鼠粪便中OVA特异性sIgA在1,2,3周时分别为(19.39±2.89),(17.60±5.65),(14.48±4.55)μg·L-1,茯苓多糖组为(28.44±3.72),(42.24±5.14),(26.29±4.82)μg·L-1,与对照组比较有统计学意义(P<0.05)。对照组小鼠派氏结B细胞CD80,CD86表达率(%)分别为1.84±0.11和16.50±1.47;茯苓多糖组表达为2.76±0.29,22.02±1.89,高于对照组,有统计学意义(P<0.05)。结论:茯苓多糖对肠道sIgA分泌具有调节作用,这与其活化派氏结B淋巴细胞有关。
Objective: To observe the effect of tuckahoe polysaccharide on the intestinal mucosal immune response induced by ovalbumin (OVA) in mice and to explore its regulation on mucosal immunity and its related mechanism. Methods: BALB / c mice were randomly divided into 2 groups: control group and tuckahoe polysaccharide group. The control group was orally administered with equal volume of normal saline. The tuckahoe polysaccharide group was orally administered once daily by body weight at a dose of 200 mg · kg -1 One week after administration, 5 mice in each group were orally immunized with 5 mg of oral antigen ovalbumin (OVA). Two weeks after the immunization, mice stool samples were collected and ELISA The OVA-specific secreted immunoglobulin A (sIgA) was detected in feces of mice. One week after administration, the mice were sacrificed and the B lymphocytes in Peyer’s patches were examined by flow cytometry Costimulatory molecules CD80, CD86 expression. Results: The OVA-specific sIgA in the feces of the control group was (19.39 ± 2.89), (17.60 ± 5.65) and (14.48 ± 4.55) μg · L-1 at 1, 28.44 ± 3.72), (42.24 ± 5.14) and (26.29 ± 4.82) μg · L-1, respectively, which were statistically significant compared with the control group (P <0.05). The expression rates of CD80 and CD86 in B cells of mice in control group were 1.84 ± 0.11 and 16.50 ± 1.47, respectively. The expression of tuckahoe polysaccharide in group B was 2.76 ± 0.29 and 22.02 ± 1.89, respectively, which was higher than that in control group P <0.05). Conclusion: Tuckahoe polysaccharide has a regulatory effect on intestinal sIgA secretion, which is related to activation of B lymphocytes.