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目的:观察海洋放线菌素X2对柯萨奇病毒B3(CVB3)感染ECV304细胞细胞间黏附分子1(ICAM-1)表达的影响。方法:采用MTT法检测不同浓度的海洋放线菌素X2作用病毒后的抑制情况;采用RT-PCR和流式细胞术分别测定CVB3感染的ECV304细胞在海洋放线菌素X2作用前后不同时间点的ICAM-1的mRNA和蛋白的表达水平。结果:CVB3感染ECV304细胞后,ICAM-1蛋白表达在12 h～54 h显著高于正常ECV304细胞对照组(P<0.05);海洋放线菌素X2干预后,于12 h～54 h降低ICAM-1蛋白的表达,与CVB3感染组相比差异有统计学意义(P<0.05);海洋放线菌素X2干预后,于24 h～54 h降低ICAM-1mRNA的表达,与CVB3感染组相比差异有统计学意义(P<0.05)。结论:海洋放线菌素X2在病毒吸附前对CVB3具有抗病毒作用,并可下调CVB3诱导的ECV304细胞ICAM-1的mRNA和蛋白的表达。
Objective: To observe the effect of Actinomycin X2 on the expression of intercellular adhesion molecule 1 (ICAM-1) in ECV304 cells infected with Coxsackievirus B3 (CVB3). Methods: MTT assay was used to detect the inhibitory effect of Actinomycin X2 treated with different concentrations of marine actinomycin X2. RT-PCR and flow cytometry were used to determine the inhibitory effect of CVB3-infected ECV304 cells at different time points Of ICAM-1 mRNA and protein expression levels. Results: The expression of ICAM-1 protein in CVB3-infected ECV304 cells was significantly higher than that in normal ECV304 cells from 12 h to 54 h (P <0.05). ICAM-1 protein expression decreased after 12 h to 54 h (P <0.05). Compared with CVB3 infection group, the expression of ICAM-1 mRNA was decreased from 24 h to 54 h after exposure to actinomycin-X2, and the expression of ICAM- The difference was statistically significant (P <0.05). CONCLUSION: Actinomycin X2 has antiviral effect on CVB3 before virus adsorption and down-regulates the expression of ICAM-1 mRNA and protein in ECV304 cells induced by CVB3.